Ankyron HLA_A0201_FLLTRILTI Clone AO40418

Flow cytometric analysis of liver cancer cell lines HepG2 and HepG2.2.15 cells (obtained from the transduction of HBV genome into HepG2). HepG2.2.15 cells were stimulated with IFN gamma to induce higher expression of MHC class I molecules, then labeled with 5 µg/50 µl Ankryron ANK03341_1_A040418 and 2.5 µl/50 µl anti-V5 PE antibody, (green). Negative controls: HepG2 with IFNgamma (blue), HepG2 without IFNgamma (pale blue), HepG2.2.15 without IFNgamma (pale green), unstained HepG2.2.15 with IFNgamma (dark grey), unstained HepG2.2.15 without IFNgamma (pale grey). Data kindly provided by Antonio Bertoletti, Duke-NUS Medical School.

Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AO40418 binding to 0.5 picomoles of immobilised HLA_A0201_FLLTRILTI, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.