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Ankyron HIF1A_MOUSE Clone EM30724
Ankyrons® Testing Data
J774A.1 mouse macrophage were cultured overnight in either media alone or in the presence of cobalt (II) chloride to stabilise HIF1α, then fixed in formaldehyde (1%), permeabilised with 0.5% Triton X-100 and stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
J774A.1 mouse macrophage were cultured overnight in either media alone or in the presence of cobalt (II) chloride to stabilise HIF1α, then permeabilised with 0.5% Triton X-100 and stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® EM30724 binding to 0.5 picomoles of immobilised HIF1A_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.