Ankyron HIF1A_HUMAN Clone AG40135

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Price £245.00
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Clone Information

Clone ?

AG40135
Gene

HIF1A BHLHE78 MOP1 PASD8
UniProtID

Q16665
Organism

Homo sapiens (Human)
Entry Name

HIF1A_HUMAN
SNR ?

161
Product Type

Monomer
Ankyron Applications

,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default

Alphalux 405

Alphalux 488
Alphalux 532
Alphalux 555
Alphalux 647
Alphalux 680
Biotin
FLAG
V5

Ankyrons® Testing Data

Flow cytometric analysis of monocytes using FOXP3 Fixation/Permeabilization Buffer Set and pre-conjugating 0.4 μg of Ankyron with anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AG40135 binding to 0.5 picomoles of immobilised HIF1A_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® AG40135 binding to HIF1A_HUMAN, of value Kd = 168.0 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.

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