Ankyron HAVR2_HUMAN Clone AB30201

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Clone Information

Clone ?

AB30201
Gene

HAVCR2 TIM3 TIMD3
UniProtID

Q8TDQ0
Organism

Homo sapiens (Human)
Entry Name

HAVR2_HUMAN
SNR ?

7811
Product Type

Monomer
Ankyron Applications

, ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
V5, His, Biotin (V5, His), FLAG (V5, His), Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His)
Ankyron Kd

Kd <10nM

Ankyrons® Testing Data

Monocyte-derived dendritic cells were stained using 2.25 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy. Inset: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AB30201 binding to 0.5 picomoles of immobilised HAVR2_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® AB30201 binding to HAVR2_HUMAN, of value Kd = 9.01 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.

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