Ankyron HARI2_HELPZ Clone CS31168

Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CS31168 binding to 0.5 picomoles of immobilised HARI2_HELPZ, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

Heat maps of Ankyrons binding to each of the helminth HpARI proteins and to some truncations of HpARI2 (left panel) and specificities of each Ankyron to the HpARI or HpBARI against which it was raised, and other members of the family (right panel). Note that binding to HpBARI_Hom2 gives the lowest signal, as this is divergent from original HpBARI. All data are from ELISAs and courtesy of Henry McSorley, University of Dundee.