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Ankyron GPC3_HUMAN Clone AE40122
Clone Information
|
Clone ?
|
AE40122 |
|
Gene
|
GPC3 OCI5 |
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UniProtID
|
P51654 |
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Organism
|
Homo sapiens (Human) |
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Entry Name
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GPC3_HUMAN |
|
SNR ?
|
10240 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, IHC, ELISA, WB |
|
Pack Size
|
100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
HepG2 were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Immunohistochemical analysis of paraffin-embedded human liver using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9).
Flow cytometric analysis of HepG2 cells following permeabilisation and staining with pre-conjugated Ankyron (0.4 μg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Colorimetric western blot analysis of purified target protein (1 µg - 10 ng) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AE40122 binding to 0.5 picomoles of immobilised GPC3_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.