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Ankyron GBP2_HUMAN Clone AK41259
Clone Information
|
Clone ?
|
AK41259 |
|
Gene
|
GBP2 |
|
UniProtID
|
P32456 |
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Organism
|
Homo sapiens (Human) |
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Entry Name
|
GBP2_HUMAN |
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SNR ?
|
732 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, IHC, ELISA |
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Pack Size
|
100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
Dendritic cells, stimulated with LPS overnight, were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Dendritic cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Immunohistochemical analysis of paraffin-embedded human spleen using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9).
Flow cytometric analysis of human DCs, either immature (red histogram) or following LPS-induced maturation (yellow histogram), following permeabilization and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC. Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AK41259 binding to 0.5 picomoles of immobilised GBP2_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.