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Ankyron G3P_HUMAN Clone DP40478
Clone Information
|
Clone ?
|
DP40478 |
|
Gene
|
GAPDH GAPD CDABP0047 OK/SW-cl.12 |
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UniProtID
|
P04406 |
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Organism
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Homo sapiens (Human) |
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Entry Name
|
G3P_HUMAN |
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SNR ?
|
2642 |
|
Product Type
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Monomer |
|
Ankyron Applications
|
IF, IHC, ELISA, Kd |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
|
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Ankyron Kd
|
Kd Available |
Ankyrons® Testing Data
MCF7 were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Immunohistochemical analysis of paraffin-embedded human liver using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica EDTA buffer (pH9), 40 min.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® DP40478 binding to 0.5 picomoles of immobilised G3P_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® DP40478 binding to G3P_HUMAN, of value Kd = 102.0 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.