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Ankyron FZD10_HUMAN Clone AJ30326
Clone Information
|
Clone ?
|
AJ30326 |
|
Gene
|
FZD10 |
|
UniProtID
|
Q9ULW2 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
FZD10_HUMAN |
|
SNR ?
|
306 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
HCT116 were fixed in formaldehyde (1%), permeabilised with 0.5% Triton X-100 then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of HCT116 cells following permeabilisation with FOXP3 nuclear permeabilisation buffer and staining with pre-conjugated Ankyron (0.4 μg) with anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AJ30326 binding to 0.5 picomoles of immobilised FZD10_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.