Ankyron FGFR3_MOUSE Clone AM40164

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Price £245.00
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Clone Information

Clone ?

AM40164
Gene

Fgfr3 Mfr3 Sam3
UniProtID

Q61851
Organism

Mus musculus (Mouse)
Entry Name

FGFR3_MOUSE
SNR ?

12266
Product Type

Monomer
Ankyron Applications
IF, ELISA
Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His)
Alphalux 488 (V5, His)
Alphalux 532 (V5, His)
Alphalux 555 (V5, His)
Alphalux 647 (V5, His)
Alphalux 680 (V5, His)
Biotin (V5, His)
FLAG (V5, His)
V5, His

Ankyrons® Testing Data

Mouse macrophage J774A.1 cells (FGFR3-positive) and HUVEC (FGFR3-negative) were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AM40164 binding to 0.5 picomoles of immobilised FGFR3_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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