Ankyron FGF23_HUMAN Clone BV40886

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Price £245.00
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Clone Information

Clone ?

BV40886

Gene

FGF23 HYPF UNQ3027/PRO9828

UniProtID

Q9GZV9

Organism

Homo sapiens (Human)

Entry Name

FGF23_HUMAN

SNR ?

5598

Product Type

Monomer

Ankyron Applications

, ,

Pack Size

100ug

Label ?
Clones are also labelled with a His tag by default

Alphalux 405

Alphalux 488
Alphalux 532
Alphalux 555
Alphalux 647
Alphalux 680
Biotin
FLAG
V5

HepG2 were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin),
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BV40886 binding to 0.5 picomoles of immobilised FGF23_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Colorimetric western blot analysis of purified target protein (1µg - 100 ng) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).

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