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Ankyron FCGR1_HUMAN Clone BV40367
Clone Information
|
Clone ?
|
BV40367 |
|
Gene
|
FCGR1A FCG1 FCGR1 IGFR1 |
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UniProtID
|
P12314 |
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Organism
|
Homo sapiens (Human) |
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Entry Name
|
FCGR1_HUMAN |
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SNR ?
|
119 |
|
Product Type
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Monomer |
|
Ankyron Applications
|
F, IHC, ELISA, Kd |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
|
|
Ankyron Kd
|
Kd <100nM |
Ankyrons® Testing Data
Immunohistochemical analysis of paraffin-embedded human spleen using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9), 40 min.
Immunohistochemical analysis of paraffin-embedded human spleen using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9), 40 min.
Flow cytometric analysis of human monocytes following pre-conjugation of 0.33 μg of Ankyron with anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BV40367 binding to 0.5 picomoles of immobilised FCGR1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® BV40367 binding to FCGR1_HUMAN, of value Kd = 45.5 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.