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Ankyron FCG3B_HUMAN Clone AZ40416
Clone Information
|
Clone ?
|
AZ40416 |
|
Gene
|
FCGR3B CD16B FCG3 FCGR3 IGFR3 |
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UniProtID
|
O75015 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
FCG3B_HUMAN |
|
SNR ?
|
1342 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA, Kd |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
|
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Ankyron Kd
|
Kd <100nM |
Ankyrons® Testing Data
Monocyte-derived Dendritic cells were fixed in formaldehyde (4%), then stained using 2.5 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy. Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of human monocytes following co-incubation of 0.22 μg Ankyron with anti-V5 APC (red). Negative control, anti-V5 APC only.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AZ40416 binding to 0.5 picomoles of immobilised FCG3B_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® AZ40416 binding to FCG3B_HUMAN, of value Kd = 20.1 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.