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Ankyron FABPI_HUMAN Clone AK40455
Clone Information
|
Clone ?
|
AK40455 |
|
Gene
|
FABP2 FABPI |
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UniProtID
|
P12104 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
FABPI_HUMAN |
|
SNR ?
|
7778 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA, WB |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
HCT116 were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of permeabilised HCT116 following pre-conjugation of 0.4 μg of Ankyron with anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Colorimetric western blot of recombinant protein, using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP antibody. We cannot guarantee that this Ankyron will detect protein within cell lysate.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AK40455 binding to 0.5 picomoles of immobilised FABPI_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.