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Ankyron ETV7_HUMAN Clone BV40265
Clone Information
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Clone ?
|
BV40265 |
|
Gene
|
ETV7 TEL2 TELB TREF |
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UniProtID
|
Q9Y603 |
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Organism
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Homo sapiens (Human) |
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Entry Name
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ETV7_HUMAN |
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SNR ?
|
425 |
|
Product Type
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Monomer |
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Ankyron Applications
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F, IF, IHC, ELISA, WB |
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Pack Size
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100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
HeLa were fixed in formaldehyde (1%), permeabilised with 0.5% Triton X-100 then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Immunohistochemical analysis of paraffin-embedded colorectal cancer using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9), 40 min.
Flow cytometric analysis of human HeLa following permeabilization and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Colorimetric western blot analysis of purified target protein (1µg - 100 ng) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BV40265 binding to 0.5 picomoles of immobilised ETV7_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.