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Ankyron ERBB3_HUMAN Clone AF40429
Ankyrons® Testing Data
HeLa cells were fixed for 5 min in 4 % formaldehyde then stained using 10 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilization Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AF40429 binding to 0.5 picomoles of immobilised ERBB3_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.