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Ankyron EPHB4_HUMAN Clone AE40489
Clone Information
|
Clone ?
|
AE40489 |
|
Gene
|
EPHB4 HTK MYK1 TYRO11 |
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UniProtID
|
P54760 |
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Organism
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Homo sapiens (Human) |
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Entry Name
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EPHB4_HUMAN |
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SNR ?
|
131579 |
|
Product Type
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Monomer |
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Ankyron Applications
|
F, IF, ELISA, WB |
|
Pack Size
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100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
MCF-7 were stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of MCF7 following fixation with formaldehyde (1%) and subsequent co-incubation of 0.4 μg of Ankyron with anti-V5 APC (red). Negative control, anti-V5 apc only (gray).
Colorimetric western blot analysis of purified target protein (1µg - 100 ng) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AE40489 binding to 0.5 picomoles of immobilised EPHB4_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.