Ankyron EPHB2_HUMAN Clone AE50270

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Price £245.00
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Clone Information

Clone ?

AE50270
Gene

EPHB2 DRT EPHT3 EPTH3 ERK HEK5 TYRO5
UniProtID

P29323
Organism

Homo sapiens (Human)
Entry Name

EPHB2_HUMAN
SNR ?

7386
Product Type

Monomer
Ankyron Applications
IF, ELISA
Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
V5, His
Biotin (V5, His)
FLAG (V5, His)
Alphalux 405 (V5, His)
Alphalux 488 (V5, His)
Alphalux 532 (V5, His)
Alphalux 555 (V5, His)
Alphalux 647 (V5, His)
Alphalux 680 (V5, His)

Ankyrons® Testing Data

HCT116 were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AE50270 binding to 0.5 picomoles of immobilised EPHB2_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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