Ankyron EPCAM_MOUSE Clone AT40784

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Price £245.00
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Clone Information

Clone ?

AT40784
Gene

Epcam Tacstd1
UniProtID

Q99JW5
Organism

Mus musculus (Mouse)
Entry Name

EPCAM_MOUSE
SNR ?

557
Product Type

Monomer
Ankyron Applications

,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His), Biotin (V5, His), FLAG (V5, His), V5, His

Ankyrons® Testing Data

Mouse macrophage J774A.1 cells were either fixed in formaldehyde (4%) and stained using 1.4 µg V5-tagged Ankyron in Staining Buffer (RPMI 1640 + 10 % human serum), (upper panel), or left unfixed and stained with Ankyron in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), (lower panel). Bound Ankyron was detected with anti-V5 antibody conjugated with APC (red) and nuclei counterstained with DAPI (blue). Image captured by epifluorescence microscopy. Inset: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AT40784 binding to 0.5 picomoles of immobilised EPCAM_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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