Ankyron EPCAM_HUMAN Clone AT30329

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Price £245.00
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Clone Information

Clone ?

AT30329
Gene

EPCAM GA733-2 M1S2 M4S1 MIC18 TACSTD1 TROP1
UniProtID

P16422
Organism

Homo sapiens (Human)
Entry Name

EPCAM_HUMAN
SNR ?

79
Product Type

Monomer
Ankyron Applications

, ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His), Biotin (V5, His), FLAG (V5, His), V5, His

Ankyrons® Testing Data

HCT116 cells were fixed in formaldehyde (4%), then stained using 5 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy. Inset: negative control of anti-V5 APC antibody alone.
Immunohistochemical analysis of paraffin-embedded human sigmoid colon tissue using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1:500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AT30329 binding to 0.5 picomoles of immobilised EPCAM_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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