Ankyron ENSA_HUMAN Clone AF40317

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Price £245.00
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Clone Information

Clone ?

AF40317
Gene

ENSA
UniProtID

O43768
Organism

Homo sapiens (Human)
Entry Name

ENSA_HUMAN
SNR ?

557
Product Type

Monomer
Ankyron Applications

,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
V5, His, Biotin (V5, His), FLAG (V5, His), Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His)

Ankyrons® Testing Data

HeLa were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AF40317 binding to 0.5 picomoles of immobilised ENSA_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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