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Ankyron ENOA_HUMAN Clone AF40519
Clone Information
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Clone ?
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AF40519 |
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Gene
|
ENO1 ENO1L1 MBPB1 MPB1 |
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UniProtID
|
P06733 |
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Organism
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Homo sapiens (Human) |
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Entry Name
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ENOA_HUMAN |
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SNR ?
|
5565 |
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Product Type
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Monomer |
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Ankyron Applications
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IF, ELISA, WB |
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Pack Size
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100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
HeLa were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Colorimetric western blot analysis of lysates from HeLa and HCT116 cells using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AF40519 binding to 0.5 picomoles of immobilised ENOA_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.