Ankyron ELAF_HUMAN Clone BJ40186

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Price £245.00
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Clone Information

Clone ?

BJ40186
Gene

PI3 WAP3 WFDC14
UniProtID

P19957
Organism

Homo sapiens (Human)
Entry Name

ELAF_HUMAN
SNR ?

3245
Product Type

Monomer
Ankyron Applications
IF, ELISA
Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His)
Alphalux 488 (V5, His)
Alphalux 532 (V5, His)
Alphalux 555 (V5, His)
Alphalux 647 (V5, His)
Alphalux 680 (V5, His)
Biotin (V5, His)
FLAG (V5, His)
V5, His

Ankyrons® Testing Data

HeLa cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BJ40186 binding to 0.5 picomoles of immobilised ELAF_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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