Ankyron EGLN_HUMAN Clone AY40419

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Price €292.00
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Clone Information

Clone ?

AY40419
Gene

ENG END
UniProtID

P17813
Organism

Homo sapiens (Human)
Entry Name

EGLN_HUMAN
SNR ?

8858
Product Type

Monomer
Ankyron Applications

, ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His), Biotin (V5, His), FLAG (V5, His), V5, His

Ankyrons® Testing Data

HUVEC (Endoglin-positive) and HCT116 (Endoglin-negative) were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of HUVECs following fixation with formaldehyde (1%) and subsequent co-incubation of 0.05 μg Ankyron with anti-V5 APC (red). Negative control, anti-V5 APC only.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AY40419 binding to 0.5 picomoles of immobilised EGLN_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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