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Ankyron EGLN_HUMAN Clone AY40422
Clone Information
|
Clone ?
|
AY40422 |
|
Gene
|
ENG END |
|
UniProtID
|
P17813 |
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Organism
|
Homo sapiens (Human) |
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Entry Name
|
EGLN_HUMAN |
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SNR ?
|
4786 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA, WB |
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Pack Size
|
100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
HUVEC (Endoglin-positive) and HCT116 (Endoglin-negative) were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
HUVEC were stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Flow cytometric analysis of HUVEC following staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Flow cytometric analysis of HUVEC following fixation in formaldehyde and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Flow cytometric analysis of HUVECs following fixation with formaldehyde (1%) and subsequent co-incubation of 0.05 μg Ankyron with anti-V5 APC (red). Negative control, anti-V5 APC only.
Colorimetric western blot analysis of purified target protein (1 µg) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AY40422 binding to 0.5 picomoles of immobilised EGLN_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.