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Ankyron EGFR_HUMAN Clone BJ40529
Clone Information
|
Clone ?
|
BJ40529 |
|
Gene
|
EGFR ERBB ERBB1 HER1 |
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UniProtID
|
P00533 |
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Organism
|
Homo sapiens (Human) |
|
Entry Name
|
EGFR_HUMAN |
|
SNR ?
|
1849 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
IF, ELISA, Kd |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
|
|
Ankyron Kd
|
Kd Available |
Ankyrons® Testing Data
HeLa cells were fixed for 5 min in 4 % formaldehyde then stained using 10 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BJ40529 binding to 0.5 picomoles of immobilised EGFR_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® BJ40529 binding to EGFR_HUMAN, of value Kd = 868.0 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.