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Ankyron EFNA5_HUMAN Clone AH50163
Clone Information
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Clone ?
|
AH50163 |
|
Gene
|
EFNA5 EPLG7 LERK7 |
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UniProtID
|
P52803 |
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Organism
|
Homo sapiens (Human) |
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Entry Name
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EFNA5_HUMAN |
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SNR ?
|
1604 |
|
Product Type
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Monomer |
|
Ankyron Applications
|
F, IF, ELISA, Kd, WB |
|
Pack Size
|
100ug |
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Label ?
Clones are also labelled with a His tag by default
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|
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Ankyron Kd
|
Kd <100nM |
Ankyrons® Testing Data
HeLa were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of permeabilised HeLa following pre-conjugation of 0.4 μg of Ankyron with anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Colorimetric western blot of recombinant protein, using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP antibody. We cannot guarantee that this Ankyron will detect protein within cell lysate.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AH50163 binding to 0.5 picomoles of immobilised EFNA5_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® AH50163 binding to EFNA5_HUMAN, of value Kd = 98.5 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.