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Ankyron DSG2_HUMAN Clone FK40173
Clone Information
|
Clone ?
|
FK40173 |
|
Gene
|
DSG2 CDHF5 |
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UniProtID
|
Q14126 |
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Organism
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Homo sapiens (Human) |
|
Entry Name
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DSG2_HUMAN |
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SNR ?
|
821 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA, WB |
|
Pack Size
|
100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
HepG2 cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue).
HepG2 cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue).
HepG2 cells were stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue).
Flow cytometric analysis of human HepG2 cells following fixation in formaldehyde and staining with pre-conjugated Ankyron (0.33 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Flow cytometric analysis of human HepG2 cells following staining with pre-conjugated Ankyron (0.33 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Colorimetric western blot analysis of purified target protein (1 µg) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® FK40173 binding to 0.5 picomoles of immobilised DSG2_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.