Ankyron CSF1R_HUMAN Clone AW40224

Immature human dendritic cells were fixed in formaldehyde (4 %) then stained using 10 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy.

Flow cytometric analysis of immature dendritic cells stained with 2.5 µg of Ankyron and anti-V5 APC conjugated antibody, applied simultaneously (red). Negative control: secondary antibody alone with no Ankyron present (black).

Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AW40224 binding to 0.5 picomoles of immobilised CSF1R_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.