Ankyron COF1_HUMAN Clone CS31018

Monocytes were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.

HUVEC were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.

HeLa cells were fixed in methanol, then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.

Immunohistochemical analysis of paraffin-embedded human colon using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 1000), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9).

Flow cytometric analysis of human HeLa following permeabilization and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).

Flow cytometric analysis of HeLa cells following permeabilisation then incubation with pre-conjugated Ankyron (0.4 μg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).

Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CS31018 binding to 0.5 picomoles of immobilised COF1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.