Ankyron CLM8_MOUSE Clone AY40233

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Price £245.00
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Clone Information

Clone ?

AY40233
Gene

Cd300a Clm8 Lmir1 Mcpir1
UniProtID

Q6SJQ0
Organism

Mus musculus (Mouse)
Entry Name

CLM8_MOUSE
SNR ?

78
Product Type

Monomer
Ankyron Applications

, , ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His), Biotin (V5, His), FLAG (V5, His), V5, His

Ankyrons® Testing Data

Mouse macrophage J774A.1 cells (CLM8-positive) and HUVEC (CLM8-negative) were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of mouse J774A.1 cells following incubation with preconjugated Ankyron ( 0.165 μg) and anti-V5 APC (red). Negative control, anti-V5 APC only.
Colorimetric western blot analysis of purified target protein (1µg - 10 ng) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AY40233 binding to 0.5 picomoles of immobilised CLM8_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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