Ankyron CLM8_MOUSE Clone AY40233

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Price £245.00
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Clone Information

Clone ?

AY40233
Gene

Cd300a Clm8 Lmir1 Mcpir1
UniProtID

Q6SJQ0
Organism

Mus musculus (Mouse)
Entry Name

CLM8_MOUSE
SNR ?

78
Product Type

Monomer
Ankyron Applications

, , ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default

Alphalux 405

Alphalux 488
Alphalux 532
Alphalux 555
Alphalux 647
Alphalux 680
Biotin
FLAG
V5

Ankyrons® Testing Data

Mouse macrophage J774A.1 cells (CLM8-positive) and HUVEC (CLM8-negative) were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of mouse J774A.1 cells following incubation with preconjugated Ankyron ( 0.165 μg) and anti-V5 APC (red). Negative control, anti-V5 APC only.
Colorimetric western blot analysis of purified target protein (1µg - 10 ng) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AY40233 binding to 0.5 picomoles of immobilised CLM8_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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