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Ankyron CLH1_HUMAN Clone BZ30530
Clone Information
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Clone ?
|
BZ30530 |
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Gene
|
CLTC CLH17 CLTCL2 KIAA0034 |
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UniProtID
|
Q00610 |
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Organism
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Homo sapiens (Human) |
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Entry Name
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CLH1_HUMAN |
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SNR ?
|
4386 |
|
Product Type
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Monomer |
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Ankyron Applications
|
F, IF, ELISA, Kd |
|
Pack Size
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100ug |
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Label ?
Clones are also labelled with a His tag by default
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|
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Ankyron Kd
|
Kd <100nM |
Ankyrons® Testing Data
HeLa cells were stained using 10 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy.
Live HeLa cells were incubated with 1.4 µg V5-tagged Ankyron fluorescently labeled with AlexaFluor 488 using Lightning Link (green) in normal cell culture medium (RPMI 1640 + 10 % human serum), then imaged by a Nikon Ji widefield microscope for 12 h, with out-of-focus blur removed by Clarify in NIS-Elements software.
HUVEC were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue) and F-actin with Phalloidin. Inset: negative control of anti-V5 APC antibody alone and F-actin staining.
HeLa cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Flow cytometric analysis of human HeLa cells following permeabilization and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BZ30530 binding to 0.5 picomoles of immobilised CLH1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® BZ30530 binding to CLH1_HUMAN, of value Kd = 57.9 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.