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Ankyron CHODL_MOUSE Clone AZ40859
Clone Information
|
Clone ?
|
AZ40859 |
|
Gene
|
Chodl |
|
UniProtID
|
Q9CXM0 |
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Organism
|
Mus musculus (Mouse) |
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Entry Name
|
CHODL_MOUSE |
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SNR ?
|
1206 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA |
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Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
Lymphocytes were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Lymphocytes were stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of human lymphocytes following permeabilization and staining with pre-conjugated Ankyron (0.33 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AZ40859 binding to 0.5 picomoles of immobilised CHODL_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.