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Ankyron CH60_HUMAN Clone AM40250
Clone Information
|
Clone ?
|
AM40250 |
|
Gene
|
HSPD1 HSP60 |
|
UniProtID
|
P10809 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
CH60_HUMAN |
|
SNR ?
|
3588 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
IF, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
HCT116 cells were fixed in formaldehyde (4%), then stained using 5 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy. Inset: negative control of anti-V5 APC antibody alone.
HUVEC were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue) and F-actin with Phalloidin. Inset: negative control of anti-V5 APC antibody alone and F-actin staining.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AM40250 binding to 0.5 picomoles of immobilised CH60_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.