Ankyron CEAM3_HUMAN Clone DR40390

Product Type
Pack Size
Select Label Clear All
Price £245.00
Quantity

Clone Information

Clone ?

DR40390
Gene

CEACAM3 CD66D CGM1
UniProtID

P40198
Organism

Homo sapiens (Human)
Entry Name

CEAM3_HUMAN
SNR ?

342600
Product Type

Monomer
Ankyron Applications

, , ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default

Alphalux 405

Alphalux 488
Alphalux 532
Alphalux 555
Alphalux 647
Alphalux 680
Biotin
FLAG
V5

Ankyrons® Testing Data

HepG2 were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® DR40390 binding to 0.5 picomoles of immobilised CEAM3_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Immunohistochemical analysis of paraffin-embedded colorectal adenocarcinoma using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9).
Colorimetric western blot analysis of lysates from HepG2 and HCT116 cells using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).

Purchase With