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Ankyron CD99_HUMAN Clone CX40193
Clone Information
|
Clone ?
|
CX40193 |
|
Gene
|
CD99 MIC2 MIC2X MIC2Y |
|
UniProtID
|
P14209 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
CD99_HUMAN |
|
SNR ?
|
297 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
Live HeLa cells were incubated with 1.4 µg V5-tagged Ankyron and anti-V5 APC sequentially (red) in normal cell culture medium (RPMI 1640 + 10 % human serum), then imaged by a Nikon Ji widefield microscope for 12 h, with out-of-focus blur removed by Clarify in NIS-Elements software.
HeLa were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
HeLa were stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Flow cytometric analysis of human HeLa following staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Flow cytometric analysis of human HeLa following fixation in formaldehye and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CX40193 binding to 0.5 picomoles of immobilised CD99_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.