Ankyron CD86_HUMAN Clone EC30456

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Price £245.00
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Clone Information

Clone ?

EC30456
Gene

CD86 CD28LG2
UniProtID

P42081
Organism

Homo sapiens (Human)
Entry Name

CD86_HUMAN
SNR ?

64
Product Type

Monomer
Ankyron Applications

, ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His), Biotin (V5, His), FLAG (V5, His), V5, His

Ankyrons® Testing Data

Dendritic Cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of human DCs, either immature (yellow histogram) or following LPS-induced maturation (red histogram), following staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC. Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® EC30456 binding to 0.5 picomoles of immobilised CD86_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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