Your basket is currently empty!
Ankyron CD80_HUMAN Clone AB40476
Clone Information
|
Clone ?
|
AB40476 |
|
Gene
|
CD80 CD28LG CD28LG1 LAB7 |
|
UniProtID
|
P33681 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
CD80_HUMAN |
|
SNR ?
|
83333 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA, Kd |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
|
|
Ankyron Kd
|
Kd <10nM |
Ankyrons® Testing Data
LPS-matured human dendritic cells were stained using 10 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy.
Dendritic cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Flow cytometric analysis of human dendritic cells either resting (orange) or following stimulation (red) overnight with LPS, prior to staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC. Negative controls, anti-V5 APC only on unstimulated (light grey) and stimulated (dark grey) cells.
Flow cytometric analysis of human dendritic cells, either resting (orange) or following stimulation (red) overnight with LPS, prior to fixation in formaldehyde (1 %) and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC. Negative controls, anti-V5 APC only on unstimulated (light grey) and stimulated (dark grey) cells.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AB40476 binding to 0.5 picomoles of immobilised CD80_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® AB40476 binding to CD80_HUMAN, of value Kd = 7.3 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.