Ankyron CD7_MOUSE Clone AO40203

Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AO40203 binding to 0.5 picomoles of immobilised CD7_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

Flow cytometric analysis of CD8+ T-cells (CD3+CD8+) from the liver of C57BL6/J mice, 30 days post infection with Lymphocytic choriomeningitis virus Clone 13. Cells were stained with preconjugated Ankyron (1µg) and anti-V5 PE (preconjugated at 4ºC for 15 minutes), 30 minutes at 4ºC. Cells were then washed before staining for other surface antibodies and run unfixed on a 5 laser Cytek Aurora. Negative control: Fluorescence minus one, FMO, with no primary or secondary Ankyron present (blue), and a no-primary control, with anti-V5 Ankyron PE alone (red). Data courtesy of Sean Hyslop and Prof. William Harris Hudson from Baylor College of Medicine.

Flow cytometric analysis of congenically distinct endogenous CD8+ T cells (C57BL/6J, CD45.2+, grey) and either CD7 wild-type (WT, CD45.1+ CD45.2-, blue) or CD7 knockout (KO, CD45.1+CD45.2+, red) cells in mouse spleen following infection with Lymphocytic choriomeningitis virus LCMV). Staining was performed using V5-tagged Ankyron, a V5-specific nanobody recombinantly expressed as rabbit IgG-Fc fusion protein, and polyclonal anti-rabbit IgG antibodies conjugated with AlexaFluor 647. Data published in Hyslop et al. Cell Reports, Volume 44, Issue 10, 116316 and shared courtesy of Sean Hyslop and Prof. William Harris Hudson from Baylor College of Medicine.