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Ankyron CD69_HUMAN Clone CD30406
Clone Information
|
Clone ?
|
CD30406 |
|
Gene
|
CD69 CLEC2C |
|
UniProtID
|
Q07108 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
CD69_HUMAN |
|
SNR ?
|
244 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
Lymphocytes were cultured overnight in either the absence or presence of phytohemagglutinin (PHA) for stimulation, prior to flow cytometric analysis, staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC. Unstimulated cells in blue, PHA-stimulated in red and negative controls of anti-V5 APC only on unstimulated and stimulated cells in light and dark grey respectively.
Lymphocytes were cultured overnight in either the absence or presence of phytohemagglutinin (PHA) for stimulation, prior to fixation in formaldehyde (1 %) and flow cytometric analysis, staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC. Unstimulated cells in blue, PHA-stimulated in red and negative controls of anti-V5 APC only on unstimulated and stimulated cells in light and dark grey respectively.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CD30406 binding to 0.5 picomoles of immobilised CD69_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.