Ankyron CD69_HUMAN Clone AW40271

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Price £245.00
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Clone Information

Clone ?

AW40271
Gene

CD69 CLEC2C
UniProtID

Q07108
Organism

Homo sapiens (Human)
Entry Name

CD69_HUMAN
SNR ?

248
Product Type

Monomer
Ankyron Applications

, ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His), Biotin (V5, His), FLAG (V5, His), V5, His

Ankyrons® Testing Data

Lymphocytes, stimulated with IL1β, 10 ng/ml overnight, were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Lymphocytes were cultured overnight in either the absence or presence of phytohemagglutinin (PHA) for stimulation, prior to fixation in formaldehyde and flow cytometric analysis, staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC. Unstimulated cells in red, PHA-stimulated in orange and negative control of anti-V5 APC only in grey.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AW40271 binding to 0.5 picomoles of immobilised CD69_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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