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Ankyron CD5_HUMAN Clone AE40102
Clone Information
|
Clone ?
|
AE40102 |
|
Gene
|
CD5 LEU1 |
|
UniProtID
|
P06127 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
CD5_HUMAN |
|
SNR ?
|
1726 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
Monocyte-derived dendritic cells were fixed in formaldehyde (4%), then stained using 2.25 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy. Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of human Lymphocytes, following incubation with 1.25 μg V5-tagged Ankyron and detecting with anti-V5-APC, (red). Negative control: anti-V5-APC alone, with no Ankyron present (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AE40102 binding to 0.5 picomoles of immobilised CD5_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.