Ankyron CD5_HUMAN Clone AE50107

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Price £245.00
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Clone Information

Clone ?

AE50107
Gene

CD5 LEU1
UniProtID

P06127
Organism

Homo sapiens (Human)
Entry Name

CD5_HUMAN
SNR ?

1034
Product Type

Monomer
Ankyron Applications
F, IF, ELISA
Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
V5, His
Biotin (V5, His)
FLAG (V5, His)
Alphalux 405 (V5, His)
Alphalux 488 (V5, His)
Alphalux 532 (V5, His)
Alphalux 555 (V5, His)
Alphalux 647 (V5, His)
Alphalux 680 (V5, His)

Ankyrons® Testing Data

Lymphocytes were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with Alexa Fluor 488 (green) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 Alphalux 488 antibody alone.
Flow cytometric analysis of human lymphocytes, following simultaneous incubation with 0.64 μg V5-tagged Ankyron and anti-V5-APC, (red). Negative control: anti-V5-APC alone, with no Ankyron present (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AE50107 binding to 0.5 picomoles of immobilised CD5_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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