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Ankyron CD5_HUMAN Clone AE50107
Ankyrons® Testing Data
Lymphocytes were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with Alexa Fluor 488 (green) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 Alphalux 488 antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AE50107 binding to 0.5 picomoles of immobilised CD5_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Flow cytometric analysis of human lymphocytes, following simultaneous incubation with 0.64 μg V5-tagged Ankyron and anti-V5-APC, (red). Negative control: anti-V5-APC alone, with no Ankyron present (grey).