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Ankyron CD4_HUMAN Clone BA40507
Ankyrons® Testing Data
Immunohistochemical analysis of paraffin-embedded human lymph node using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9), 30 min.
Immunohistochemical analysis of paraffin-embedded human lymph node using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9), 30 min.
Immunohistochemical analysis of paraffin-embedded human lymph node using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9), 40 min.
Immunohistochemical analysis of paraffin-embedded human lymph node using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9), 40 min.
Flow cytometric analysis of human Lymphocytes, following incubation with 2.5 μg V5-tagged Ankyron and detecting with anti-V5-APC, (red). Negative control: anti-V5-APC alone, with no Ankyron present (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BA40507 binding to 0.5 picomoles of immobilised CD4_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.