Ankyron CD4_HUMAN Clone AA40112

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Clone Information

Clone ?

AA40112
Gene

CD4
UniProtID

P01730
Organism

Homo sapiens (Human)
Entry Name

CD4_HUMAN
SNR ?

12418
Product Type

Monomer
Ankyron Applications

, ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default

Alphalux 405

Alphalux 488
Alphalux 532
Alphalux 555
Alphalux 647
Alphalux 680
Biotin
FLAG
V5

Ankyrons® Testing Data

Lymphocytes were stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Lymphocytes were stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with Alphalux 488 (green) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 Alphalux 488 antibody alone.
Lymphocytes were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue).
Lymphocytes were stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue).
Flow cytometric analysis of Lymphocytes following staining with pre-conjugated Ankyron (0.3 μg) with anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Human peripheral blood leukocytes were stained with a CD45-specific antibody directly conjugated with FITC and 0.4 μg V5-tagged Ankyron pre-conjugated with anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Flow cytometric analysis of human Lymphocytes following staining with pre-conjugated Ankyron (0.33 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Flow cytometric analysis of human Lymphocytes following fixation in formaldehye and staining with pre-conjugated Ankyron (0.33 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AA40112 binding to 0.5 picomoles of immobilised CD4_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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