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Ankyron CD4_HUMAN Clone AA40112
Clone Information
|
Clone ?
|
AA40112 |
|
Gene
|
CD4 |
|
UniProtID
|
P01730 |
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Organism
|
Homo sapiens (Human) |
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Entry Name
|
CD4_HUMAN |
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SNR ?
|
12418 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
Lymphocytes were stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Lymphocytes were stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with Alphalux 488 (green) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 Alphalux 488 antibody alone.
Lymphocytes were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue).
Lymphocytes were stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue).
Flow cytometric analysis of Lymphocytes following staining with pre-conjugated Ankyron (0.3 μg) with anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Human peripheral blood leukocytes were stained with a CD45-specific antibody directly conjugated with FITC and 0.4 μg V5-tagged Ankyron pre-conjugated with anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Flow cytometric analysis of human Lymphocytes following staining with pre-conjugated Ankyron (0.33 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Flow cytometric analysis of human Lymphocytes following fixation in formaldehye and staining with pre-conjugated Ankyron (0.33 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AA40112 binding to 0.5 picomoles of immobilised CD4_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.