Ankyron CD44_HUMAN Clone CS31026

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Price £245.00
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Clone Information

Clone ?

CS31026

Gene

CD44 LHR MDU2 MDU3 MIC4

UniProtID

P16070

Organism

Homo sapiens (Human)

Entry Name

CD44_HUMAN

SNR ?

2368

Product Type

Monomer

Ankyron Applications

,

Pack Size

100ug

Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His), Biotin (V5, His), FLAG (V5, His), V5, His
Ankyron Kd

Kd Available

HeLa cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CS31026 binding to 0.5 picomoles of immobilised CD44_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® CS31026 binding to CD44_HUMAN, of value Kd = 107.0 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.

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