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Ankyron CD40L_HUMAN Clone AB40467
Clone Information
|
Clone ?
|
AB40467 |
|
Gene
|
CD40LG CD40L TNFSF5 TRAP |
|
UniProtID
|
P29965 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
CD40L_HUMAN |
|
SNR ?
|
358 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IHC, ELISA, Kd |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
|
|
Ankyron Kd
|
Kd <1nM |
Ankyrons® Testing Data
Immunohistochemical analysis of paraffin-embedded human tonsil using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9), 40 min.
Flow cytometric analysis of lymphocytes following pre-conjugation of 0.4 μg of Ankyron with anti-V5 APC (red). Negative control, anti-V5 APC only.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AB40467 binding to 0.5 picomoles of immobilised CD40L_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® AB40467 binding to CD40L_HUMAN, of value Kd = 0.314 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.