Ankyron CD34_HUMAN Clone AR40148

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Price £245.00
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Clone Information

Clone ?

AR40148

Gene

CD34

UniProtID

P28906

Organism

Homo sapiens (Human)

Entry Name

CD34_HUMAN

SNR ?

290

Product Type

Monomer

Ankyron Applications
IF, ELISA
Pack Size

100ug

Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His)
Alphalux 488 (V5, His)
Alphalux 532 (V5, His)
Alphalux 555 (V5, His)
Alphalux 647 (V5, His)
Alphalux 680 (V5, His)
Biotin (V5, His)
FLAG (V5, His)
V5, His
HUVEC were stained using 2.5 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy. Inset: negative control of anti-V5 APC antibody alone.
HUVEC were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AR40148 binding to 0.5 picomoles of immobilised CD34_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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