Ankyron CD28_HUMAN Clone AB40308

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Price £245.00
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Clone Information

Clone ?

AB40308
Gene

CD28
UniProtID

P10747
Organism

Homo sapiens (Human)
Entry Name

CD28_HUMAN
SNR ?

7222
Product Type

Monomer
Ankyron Applications
IF, ELISA
Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
V5, His
Biotin (V5, His)
FLAG (V5, His)
Alphalux 405 (V5, His)
Alphalux 488 (V5, His)
Alphalux 532 (V5, His)
Alphalux 555 (V5, His)
Alphalux 647 (V5, His)
Alphalux 680 (V5, His)

Ankyrons® Testing Data

Lymphocytes were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with Alphalux 488 (green) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 Alphalux 488 antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AB40308 binding to 0.5 picomoles of immobilised CD28_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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