Ankyron CD244_HUMAN Clone AC40190

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Price £245.00
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Clone Information

Clone ?

AC40190

Gene

CD244 2B4

UniProtID

Q9BZW8

Organism

Homo sapiens (Human)

Entry Name

CD244_HUMAN

SNR ?

707

Product Type

Monomer

Ankyron Applications
F, ELISA
Pack Size

100ug

Label ?
Clones are also labelled with a His tag by default
V5, His
Biotin (V5, His)
FLAG (V5, His)
Alphalux 405 (V5, His)
Alphalux 488 (V5, His)
Alphalux 532 (V5, His)
Alphalux 555 (V5, His)
Alphalux 647 (V5, His)
Alphalux 680 (V5, His)
Lymphocytes were cultured overnight in either the absence or presence of phytohemagglutinin (PHA) for stimulation, prior to fixation in formaldehyde (1 %) and flow cytometric analysis, staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC. Unstimulated cells in blue, PHA-stimulated in red and negative controls of anti-V5 APC only on unstimulated and stimulated cells in light and dark grey respectively.
Flow cytometric analysis of human lymphocytes following fixation in formaldehye and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AC40190 binding to 0.5 picomoles of immobilised CD244_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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